Fig. 5
Expression of LD2-LD3-LD4 leads to an increase in number and size of FAs and a reduction of FA turnover a) Confocal images of PFA-fixed HeLa cells transfected with GFP LD2-LD3-LD4 and immunostained for Vinculin. Cells expressing GFP LD2-LD3-LD4 (marked with asterisk) form more and larger FAs, that are localized more ventrally. (b-c) Quantification of the total number of FAs per cell (b) and mean FA size (c), confirm significant increase of FA number (39.41 ± 4.48 FAs, n = 27 control cells compared to 66.17 ± 6.35 FAs, n = 29 GFP LD2-LD3-LD4 expressing cells) and size (4.29 ± 0.21 μm2, n = 526 FAs from 27 control cells, compared to 8.83 ± 0.33 μm2, n = 915 FAs from 29 GFP LD2-LD3-LD4 expressing cells) and. d) Overlayed confocal images from time lapse recordings, showing FA turnover in control and GFP LD2-LD3-LD4 expressing cells. Cells were imaged over a period of 25 min and FAs were visualized using RFP Vinculin. e) Quantification of the FA turnover rate assessed as the percentage change in the average intensity of selected FAs over time. FAs in GFP LD2-LD3-LD4 expressing cells display significantly slower turnover rates (33.77% ± 4.86 change at 25 min, n = 52 FAs) compared to control cells (79.39% ± 6.22change at 25 min, n = 38 FAs). **; p < 0.005, ***; p < 0.0001, *; p = 0.0118, ***; p ≤ 0.0005