Fig. 1

LD2-LD3-LD4 interacts with FAK directly a) Schematic representation of the LD2-LD3-LD4 polypeptide, composed of amino acids 54–279 of Paxillin, including the LD2-LD3-LD4 motifs and fused to GFP. b) Representative Western Blot (using anti-GFP) showing expression of a stable protein encoding GFP-fused LD2-LD3-LD4, in HeLa cells (expected molecular weight ~ 51 kDa). c) Confocal images of PFA-fixed HeLa cells expressing GFP LD2-LD3-LD4. The protein is primarily localized in the cytosol (Scale bar: 10 μm). d) Western blots showing immunoprecipitated GFP (left) and GFP LD2-LD3-LD4 (right), blotted for GFP and FAK. Co-precipitation of FAK (125 kDa) is only observed in HeLa cells co-expressing GFP LD2-LD3-LD4