Fig. 6
From: Lactic acid promotes metastatic niche formation in bone metastasis of colorectal cancer
The overexpression of Cadherin-11 enhances the adhesion ability of osteoclast precursors. a RT-PCR analysis tested the mRNA level of Cadherin-11 in CD115(+) precursors to verify the efficiency of Cadherin-11 siRNA. b Transwell assay showed the mobility of CD115(+) precursors after transfected with Cadherin-11 siRNA or negative control in the presence of LA or not for 12 h. c The quantification of Transwell assay in (b) by using absorbency analysis. d Immunofluorescence analysis showed Cadherin-11 expression in CD115(+) precursors after treated by LA for 72 h (left), the percentage of Cadherin-11 and CD115 dual-positive cells in total cells was calculated (middle) and the quantification of adhesive CD115(+) precursors (right). *p < 0.05, **p < 0.01, ***p < 0.001