Fig. 4
From: Lactic acid promotes metastatic niche formation in bone metastasis of colorectal cancer
The activation of PI3K-AKT-CREB pathway at least partly regulates the expression of CXCL10 in osteoclast precursors. a GO ontology analysis showed the enriched pathways in transcriptomic profiling in LA-treated group comparing with control group. b Western blotting analysis showed the activation of PI3K-AKT pathways, including the total protein levels and phosphorylated levels of mTOR, CREB, AKT and PI3K. c RT-PCR analysis and Western blotting showed the mRNA expression and protein expression of CXCL10 in CD115(+) precursors treated by LA with/without each antagonist of PI3K-AKT pathway for 72 h in vitro. e Conditional medium was collected from CD115(+) precursors after stimulated by LA with/without each antagonist of PI3K-AKT pathway, then CD4(+) T cells were treated by conditional medium for 48 h. RT-PCR analysis and Western blot detected the mRNA expression and protein level of RANKL in each group. *p < 0.05, **p < 0.01, ***p < 0.001