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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Lysophosphatidic acid-RAGE axis promotes lung and mammary oncogenesis via protein kinase B and regulating tumor microenvironment

Fig. 4

LPA-RAGE axis mediates cellular proliferation, migration and invasion via PKB signaling pathway in mammary cancer cells. a, b Wound healing assay showing the migration of RAGE silenced MDA MB-231 and MCF-7 cells pre-treated with PKB inhibitor (LY294002) followed by no treatment (control), LPA and 5% FBS treatment. c, d Trans-well invasion of RAGE silenced MDA MB-231 and MCF-7 cells pre-treated with PKB inhibitor (LY294002) followed by no treatment (control), LPA and 5% FBS treatment with their quantitative representations. e Immunoblots showing the c-Myc, Slug, Twist, and GAPDH expression in control shRNA and RAGE shRNA treated MDA MB-231 and MCF-7 cells with and without LPA stimulation. f Quantification of cell proliferation assay of control and RAGE silenced MDA MB-231 and MCF-7 with LPA post PKB inhibitor treatment (LY294002). For all experiments, data are means ± SD., n = 3–7. * ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001

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Cell Communication and Signaling

ISSN: 1478-811X