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Fig. 8 | Cell Communication and Signaling

Fig. 8

From: Clathrin- and dynamin-dependent endocytosis limits canonical NF-κB signaling triggered by lymphotoxin β receptor

Fig. 8

Clathrin and dynamin deficiency reduces activation of the non-canonical NF-κB pathway. a-c A549, HEK293T and CCD1070Sk cells were transfected with siRNAs targeting clathrin (CHC) (two oligonucleotides) along with control, non-targeting siRNAs (two oligonucleotides) and stimulated or not with Ago for 1 h. d, f A549 cells were transfected with siRNAs targeting dynamin-1/2 (three combinations of oligonucleotides targeting dynamin-1 and dynamin-2, see Methods) along with non-targeting control (Ctrl) siRNAs (two combinations of oligonucleotides, see Methods) and stimulated or not with Ago for 1 h. e, g A549 cells were treated with dynasore (DYN) or DMSO and stimulated or not with Ago for 1 h. Lysates of cells were analyzed by Western blotting with antibodies against the indicated proteins. Representative blots are shown. Values presented below blots represent the averaged p52/p100/loading control (a-e) or NIK/loading control ratio (f-g) from at least three experiments (normalized to the selected control, set as 1) in cells stimulated with Ago

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Cell Communication and Signaling

ISSN: 1478-811X