Fig. 2

Ligand-bound LTβR localizes to endocytic compartments. a A549 cells were stimulated with Ago for the indicated time periods and immunostained for ligand-bound LTβR, EEA1 and LAMP1. Insets show magnified views of boxed regions in the main images. Scale bars, 20 μm. b Graphs represent the analysis of integral intensity and number of LTβR vesicles, and colocalization between LTβR and EEA1 or LAMP1. Data represent the means ± SEM, n = 4; ns - P > 0.05; *P ≤ 0.05; **P ≤ 0.01 by one sample t test