Fig. 3

Effect of CGRP and SP on ECs transcriptional profiles. ECs cultivated in 48-wells plates were treated either with CGRP (from 0,1 nM to 100 nM) or SP (from 10 nM to 10 μM) for 4 days (white bars) or 7 days (grey bars) of culture. Total RNA was extracted followed by Vegfa, Angpt1, Col4, and Mmp2 gene expression analysis by RT-qPCR. VegfA expression is upregulated by CGRP after 7 days of culture whereas VegfA, Angpt1, Col4 and Mmp2 expression is upregulated by SP. (n = 3 independent experiments, One-way ANOVA followed by post hoc Dunnett’s test, * p < 0.05, ** p < 0.01, *** p < 0.001)