Fig. 3

Effects of the anti-CD47 mAb 1F7 on cytosolic Ca²⁺ levels and phosphatidylserine exposure in human red blood cells. Representative histogram (Black line: 0 μg/mL mAb 1F7, orange line: 10 μg/mL mAb 1F7, following a 4-h incubation; a) and means ± SEM. b of Fluo3-positive RBCs (%) (n = 6; b) following incubation for 0–24 h at 37 °C in the presence of 10 μg/mL mAb 1F7. ** indicate significant difference (P < 0.01) from the zero time-point. c Means ± SEM of annexin V positive RBCs (n = 3) following 24-h incubation with 0–10 μg/mL mAb 1F7. * indicates significant difference (P < 0.05) from the absence of mAb 1F7. d Means ± SEM of annexin V positive RBCs (n = 5) following incubation with 10 μg/mL mAb 1F7 for 0–24 h. * and ** indicate significant difference (P < 0.05 and P < 0.01, respectively) from the zero time-point. e Means ± SEM of annexin V positive RBCs (n = 7) following 24-h incubation with 10 μg/mL mAb 1F7 in the absence or presence of 1 mM CaCl₂