Fig. 1

Effect of thrombospondin-1 on Ca²⁺ homeostasis in human red blood cells. Representative histogram (Black line: 0 μg/mL TSP-1, orange line: 50 μg/mL TSP-1; a and means ± SEM. b of Fluo3-positive RBCs (%) (n = 7) following a 48-h incubation at 37 °C in Ringer’s solution containing 0–50 μg mL^− 1 TSP-1. *** indicate significant difference (P < 0.001) from the absence of TSP-1. c Raw current traces from a representative cell under control conditions (left panel) and in the presence of 50 μg/mL TSP-1 (right panel). For clarity, in both panels, not all traces, but every second one, starting with -100 mV, are being shown. Whole-cell currents were elicited by voltage steps from − 100 mV to 80 mV for 500 ms in 20 mV increments at 5 s intervals, Vh = − 30 mV. d I/V-curves in the absence (control; black diamonds) or in the presence of 50 μg/mL TSP-1 (red triangles; n = 11). Data are expressed as mean current ± SEM. * indicates significant difference (P < 0.05) from the absence of TSP-1. e I/V-curves in the absence (control; black triangles) or in the presence of 50 μg/mL TSP-1 and 1 mM amiloride (red squares; n = 11). Data are expressed as mean current ± SEM. * indicates significant difference (P < 0.05) from the absence of TSP-1 and amiloride