Fig. 6

Co-treatments with enzalutamide and SUMO inhibitor decrease the migration and metastatic phenotype of TamR BCa. a-b Images from scratch wound healing assays indicate that GA + Enz decrease the migration of TamR-7 cells. Black dotted lines define the scratch borders and white lines represent gap width. Remaining scratch gap opening in (b) for each treatment at each time point was calculated as a fold relative to the corresponding original gap width at 0 h. Graph is a representation of two independent experiments, each performed in duplicates. c-d Single-cell suspensions of adherent TamR-7 cells were generated, grown in nonadherent media with indicated treatments and mammosphere images were taken 7 days post treatments. First-generation mammospheres were passaged and dissociated into single cell suspensions and cultured the same way to test for second-generation spheroids. Graph in panel (c) represents total mammosphere counts as mean ± SEM from four independent experiments; representative images are shown in (Additional file 1: Fig. S5B). d Images illustrate mammosphere size and black-dashed circles highlight size differences between untreated and treated spheroids. Comparisons with control group or between groups, in graphs B and C, were assessed using one-way ANOVA followed by Tukey’s multiple comparison test and significance is illustrated on graphs. e Schematic representation of AR regulation by SUMO in ET-S and ET-R BCa. Detailed description is provided in text. Encircled S represents SUMO