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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Constitutively active androgen receptor supports the metastatic phenotype of endocrine-resistant hormone receptor-positive breast cancer

Fig. 3

AR interacts with and is a substrate for the SUMO E3 ligase Hsp27. a Ectopic induction of a hyperSUMO environment in MCF-7 cells with HA-SUMO3 increases the interaction between AR and Hsp27. b AR-Hsp27 interaction complex is enhanced endogenously in the naturally occurring hyperSUMO environment of TamR-7 cells. a-b Interactions with chromatin-bound (CB) AR were analyzed by western blot. c Immunoprecipitation of Hsp27 was performed to assess its binding with AR, Ubc9 and SUMO-2/3 in a cell-free system. d SUMOylation of AR is enhanced under in vitro conditions when recombinant Hsp27 is added. Details are found in (Additional file 1)

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Cell Communication and Signaling

ISSN: 1478-811X