Fig. 6

Early liver injury and inflammatory mediator production after Icariside I/LPS cotreatment. a-f WT female C57BL/6 mice were pretreated with LPS (2 mg/kg) through the tail vein. 2 h later, intraperitoneally Icariside I (25 mg/kg, 50 mg/kg, 100 mg/kg, n = 6) injection was conducted. 6 h after Icariside I injection, serum levels of ALT (a), AST (b), IL-1β (c), TNF-α (d) were measured by assay kit, (e, f) IL-1β (e) and caspase-1 activity (f) in the livers was detected after BCA protein quantification and normalization processing. g H&E staining. Data are expressed as the mean ± S. ^##P < 0.01, ^###P < 0.001, vs. the control. *P < 0.05, **P < 0.01, ***P < 0.01