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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Icariside I specifically facilitates ATP or nigericin-induced NLRP3 inflammasome activation and causes idiosyncratic hepatotoxicity

Fig. 5

Icariside I facilitates ATP/nigericin-induced NLRP3 inflammasome activation dependent on mitochondrial ROS production. a, b LPS-primed BMDMs were treated with Icariside I before stimulated with ATP, nigericin, SiO2. BMDMs were loaded with MitoSOX red mitochondrial superoxide indicator (Ex/Em: 510/580 nm). After staining and washing, flow cytometry was conducted to test mtROS production. c Confocal microscopy analysis in LPS-primed BMDMs treated with Icariside I (20 μM) and then left stimulated with ATP, followed by staining with Mitotracker red and DAPI. Data are represented as the mean ± SD from at least three biological samples. #p < 0.05 vs. the control group. *P < 0.05

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Cell Communication and Signaling

ISSN: 1478-811X