Fig. 2

Hepatic dysregulation of the expression of genes involved in fatty acid oxidation in Sirt3-deficient mice fed a HFD. a, Hepatic mRNA levels of genes involved in fatty acid metabolism of livers from WT and Sirt3^−/− mice fed either a standard chow or a HFD. Data are presented as the mean ± S.D. (n = 6 per group). b, autoradiograph of EMSA to show PPAR-DNA binding activity was performed with a ³²P-labeled PPRE nucleotide and crude nuclear protein extract (NE) from livers of WT and Sirt3^−/− mice fed either a standard chow or a HFD. a, p < 0.05 vs. WT mice fed a standard chow. b, p < 0.05 vs. WT mice fed a HFD. c, p < 0.05 vs. Sirt3^−/− mice fed a standard chow. Ab, antibody