Fig. 3

Knockdown of LOXL1 in RKO and HT29 cells promotes their migration and invasion in vitro. a Western blot analysis of LOXL1 knockdown in RKO and HT29 cells expressing LOXL1-targeting or control siRNA. Total GAPDH was used as a loading control. b-c Wound healing analysis upon LOXL1 knockdown in RKO and HT29 cells transiently transfected with LOXL1-targeting or scramble siRNA at 0 h, 24 h, and 48 h. Representative images (upper panel) and quantification (lower panel) are shown as indicated. Data from three independent experiments are presented as the mean ± SD. Statistical significance was assessed by unpaired t-test; ***P < 0.001. Scale bar: 100 μm. d-e Transwell migration and Matrigel invasion assays were performed in LOXL1-knockdown and control cells. Representative images (left panel) and quantification (right panel) are shown as indicated. Data from three independent experiments are presented as the mean ± SD. Statistical significance was assessed by unpaired t-test; **P < 0.01. Scale bar: 100 μm. f-g A colony formation assay was performed using RKO and HT29 cells transfected with LOXL1-targeting or scramble siRNA. Left panel: representative images, right panel: quantification analysis. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by unpaired t-test; *P < 0.05, **P < 0.01