Fig. 3
Evaluation of the binding affinity of small molecule compounds towards PVR. a Schematic representation of the double reference-subtracted analysis system. b The responses of the small molecule compounds preliminarily screened at a single concentration of 100 μM. The compounds with responses over than 0.03 nm were labeled red. c The dissociation curves and KD values of the three compounds with high affinity to PVR were shown. Data are representative of three independent experiments