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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Mitochondrial DNA drives noncanonical inflammation activation via cGAS–STING signaling pathway in retinal microvascular endothelial cells

Fig. 3

Changes in cGAS protein and mRNA levels in RMECs after mtDNA stimulation. a RMECs were treated with 1 μg of mtDNA for 1 h, 2 h, 3 h, 4 h, 5 h, 6 h, 12 h and 24 h. And the cGAS protein levels were determined by Western blot analysis; β-actin was used as the loading control. Quantitative analysis of cGAS, as determined by densitometric analysis, expressed as a ratio of β-actin. b RT–PCR was used to determine cGAS mRNA levels in RMECs treated with 1 μg of mtDNA for different times. c Western blotting analysis of cGAS protein levels in RMECs treated with different concentrations of mtDNA for 6 h. ∗P < 0.05, ∗∗P < 0.01, n = 3 in each group

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Cell Communication and Signaling

ISSN: 1478-811X