Fig. 4

StarD13 silencing increases the size of adhesion structures in lung adenocarcinoma cells and inhibits their tail detachment. a Representative micrographs of A549 cells transfected with luciferase control, Rac1 siRNA, RhoA siRNA, StarD13 siRNA or RhoA-CA (constitutively active) that were fixed and immunostained with anti-Vinculin. Scale bar is 10 μm. The graph is a quantitation (as described in the materials and methods) represented as average adhesion size in cells of each condition presented as fold difference to luciferase control. Data are the mean −/+ SEM from 15 cells/condition from 3 different experiment and *p < 0.05. b Montage of time-lapse movie (showing frames that are 4 min apart) of Luciferase siRNA- or StarD13 siRNA-transfected A549 cells undergoing random motility in serum (for 2 h). The black arrows indicate the direction of movement. Scale bar is 10 μm