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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: Yes-associated protein (YAP) induces a secretome phenotype and transcriptionally regulates plasminogen activator Inhibitor-1 (PAI-1) expression in hepatocarcinogenesis

Fig. 7

YAP and TEAD4 interact with the SERPINE1 promoter. a ChIP analysis of TEAD4 and YAP binding to the murine (Hepa1–6) and human (Sk-Hep1) SERPINE1 promoters at the indicated binding sites predicted by the JASPAR database. Schemes illustrate the potential binding sites and the downstream control site. TS – transcriptional start. Bars illustrate the relative binding capacity at the respective site in relation to the total input. b Luciferase assay after transient transfection of HEK-293 cells with pGL3-PAI-1 with and without siRNA-mediated inhibition of YAP or TEAD4 (2 siRNAs for each gene were tested). scr. - scrambled siRNA served as control. Coexpression of pRL-CMV-Renilla vector was used for normalization. c Coexpression of pGL3 (containing wildtype and mutated TEAD4 binding sites), pRL-CMV-Renilla (all samples, for normalization) and pDEST (with and without YAP) in HEK-293 cells

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Cell Communication and Signaling

ISSN: 1478-811X