Fig. 6

PAI-1 expression is regulated by the YAP/TEAD4 complex. a Real-time PCR analysis of PAI-1 mRNA levels after siRNA-mediated silencing of the known YAP-interacting transcription factors TEAD1, TEAD4 and FOXM1. Two independent siRNAs for each transcription factor were used (#1, #2) and compared to untreated (−) and scrambled siRNA-transfected SNU-182 cells (scr.). b Protein detection of secreted PAI-1 levels in the supernatant and total protein fractions derived from cultured Sk-Hep1 cells after siRNA mediated TEAD4-Inhibition. c Liver cancer cell line Sk-Hep1 was treated with increasing Verteporfin concentrations (0.25–1.0 μM) for 24 h followed by the detection of intracellular and secreted PAI-1. For b and c, signal intensity was measured and normalized to GAPDH. ‘scr’ controls represent samples that were transfected with the transfection reagent and non-specific siRNA (40 nM) with no similarity to cDNA