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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Inhibition of αvβ3 integrin impairs adhesion and uptake of tumor-derived small extracellular vesicles

Fig. 4

DisBa-01 inhibits SEV uptake in a co-culture system. a Scanning electron microscopy of MDA-MB-231 and MCF 10A cells in single and co-culture systems. Ctrl, control. DisBa-01, 1000 nM. Scale bar, 10 μm. b Effect of integrin inhibition on MDA-MB-231 cell morphology; **p < 0.01. c Representative epifluorescence images highlighting the distribution of GFP-CD63-enriched vesicles between MDA-MB-231 expressing GFP-CD63 (green) and MCF 10A (red) cells. Scale bar, 50 μm. d Effect of integrin inhibition on MDA-MB-231-GFP-CD63 cell morphology; ***p < 0.001. e Extracellular GFP-CD63-EVs from tumor to non-malignant cells. Z-stack slices from confocal acquisition show reduction of internalized and extracellular SEVs in DisBa-01 treated cells. f Orthogonal view of MCF 10A cells showing internalized GFP-CD63-SEVs and their reduction upon DisBa-01 treatment. g Effect of integrin inhibition on SEV uptake; ***p < 0.001. h Effect of integrin inhibition on extracellular GFP-CD63; ****p < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X