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Table 1 Mass spectrometry data showing the identity of the proteins precipitated with biotinylated peptides of the Syndecan-4 cytoplasmic tail

From: Syndecan-4/PAR-3 signaling regulates focal adhesion dynamics in mesenchymal cells

Protein Name Species Database Accession ID MW (Da) Peptide Counta MS & MS/MS Scoreb Peptide Sequenced Ion Scorec Scoring Thresholdd
Atypical protein kinase C isotype-specific interacting (PAR-3) Rattus norvegicus Q9Z340.1 149,356.5 36 315 177 56
Tubulin, alpha 1C Rattus norvegicus AAH78829.1 49,905.5 8 185 159 56
Tubulin beta chain 15 Rattus norvegicus Q3KRE8.1 49,904.9 9 151 115 56
Insulin-like growth factor binding protein 5 protease Rattus norvegicus AAD52683.1 51,297.7 12 227 191 56
Type I keratin KA10 Rattus norvegicus DAA04466.1 56,470.5 7 69 53 56
Actin beta Rattus norvegicus AAH63166.1 41,723.7 20 635 487 56
Heterogeneous nuclear ribonucleoprotein A2/B1/B0 Mus musculus AAN16352.1 37,379.7 11 101 58 78
Syntenin-1 Rattus norvegicus NP_114192.1 32,402.7 14 384 299 56
Streptavidin core, residues 13,139 pH 4.5, chain B Streptomyces avidinii 1SWAB2 9191.5 6 549 498 78
  1. aNumber of peptides that match the theoretical digest of the primary protein identified
  2. bQuality score of peptide-mass fingerprint matches and of MS/MS peptide fragment ion matches, if MS/MS data were generated
  3. cQuality score of only MS/MS peptide fragment ion matched, if MS/MS data were generated
  4. dSignificant score threshold. For each given database, a hit with an “MS & MS/MS Score” above the indicated value was considered a significant identification (p < 0.05). Only hits with an “MS & MS/MS Score” above this value are reported
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