Skip to main content

Table 1 Mass spectrometry data showing the identity of the proteins precipitated with biotinylated peptides of the Syndecan-4 cytoplasmic tail

From: Syndecan-4/PAR-3 signaling regulates focal adhesion dynamics in mesenchymal cells

Protein Name

Species

Database Accession ID

MW (Da)

Peptide Counta

MS & MS/MS Scoreb

Peptide Sequenced Ion Scorec

Scoring Thresholdd

Atypical protein kinase C isotype-specific interacting (PAR-3)

Rattus norvegicus

Q9Z340.1

149,356.5

36

315

177

56

Tubulin, alpha 1C

Rattus norvegicus

AAH78829.1

49,905.5

8

185

159

56

Tubulin beta chain 15

Rattus norvegicus

Q3KRE8.1

49,904.9

9

151

115

56

Insulin-like growth factor binding protein 5 protease

Rattus norvegicus

AAD52683.1

51,297.7

12

227

191

56

Type I keratin KA10

Rattus norvegicus

DAA04466.1

56,470.5

7

69

53

56

Actin beta

Rattus norvegicus

AAH63166.1

41,723.7

20

635

487

56

Heterogeneous nuclear ribonucleoprotein A2/B1/B0

Mus musculus

AAN16352.1

37,379.7

11

101

58

78

Syntenin-1

Rattus norvegicus

NP_114192.1

32,402.7

14

384

299

56

Streptavidin core, residues 13,139 pH 4.5, chain B

Streptomyces avidinii

1SWAB2

9191.5

6

549

498

78

  1. aNumber of peptides that match the theoretical digest of the primary protein identified
  2. bQuality score of peptide-mass fingerprint matches and of MS/MS peptide fragment ion matches, if MS/MS data were generated
  3. cQuality score of only MS/MS peptide fragment ion matched, if MS/MS data were generated
  4. dSignificant score threshold. For each given database, a hit with an “MS & MS/MS Score” above the indicated value was considered a significant identification (p < 0.05). Only hits with an “MS & MS/MS Score” above this value are reported