Fig. 2

Over-expression of TPX2 promotes HCC proliferation despite inhibition of Hh/GLI signaling. a-b. Validation of HepG2 cells stably over-expressing TPX2 using WB analysis with the indicated antibodies (a) and real-time PCR with the indicated primers (b). c. Comparison of the proliferative ability of Lv-control + DMSO, Lv-control + GANT61 (2 μM), Lv-TPX2 + DMSO, and Lv-TPX2 + GANT61 (2 μM) in HepG2 cells treated with EdU. Scale bar, 100 μm. d. The ratio of EdU-positive cells was quantified using the ImageJ software (n = 3). e. Cell growth curves of Lv-control + DMSO, Lv-control + GANT61 (2 μM), Lv-TPX2 + DMSO, and Lv-TPX2 + GANT61 (2 μM) in HepG2 cells. f-i. Comparison of the proliferative ability of Lv-control + DMSO, Lv-control + GANT61 (2 μM), Lv-TPX2 + DMSO, and Lv-TPX2 + GANT61 (2 μM) in HepG2 cells using soft-agar colony formation assays (f) and plate colony formation assay (h). Soft-agar colonies (g) and plate colonies (i) were counted using the ImageJ software. Scale bar, 100 μm. Data was shown as mean ± SD (n = 3). *, p < 0.05; **, p < 0.01, N.S. denotes not significant