Fig. 6

CP phosphorylation increases cell adhesion and actin disassembly. a-c PC-3 cells were transiently transfected with wild-type (WT) Capza1 and Capzb2 or their phosphodeficient (SA) or phosphomimicking (SE) mutants. Cell adhesion to collagen was recorded according to the electrical impedance. Similar experiments were performed at least three times with three parallel samples, while shown are representative results from one experiment with three parallel samples. The statistical analyses were performed pairwise for each graph. d-e Actin polymerization was performed by combining muscle actin with 5% pyrene-labelled actin. Thereafter actin disassembly was followed in vitro with or without addition of bacterially produced CPs. Actin re-assembly was prevented by vitamin D-binding protein. Shown are average results from two to four independent experiments with different combinations of samples. The statistical analyses were performed by comparing to the CP wild-type control (Capza1-WT + Capzb2-WT)