Fig. 5

PIM inhibition increases the number of actin protrusions at cell edges. PC-3 cells were treated for 24 h with 10 μM DHPCC-9, AZD-1208 or SGI-1776. Single cell tracking was performed to visualize the migration route and distance after PIM inhibition. a Shown are averages of indicated numbers (n) of cells along with b representative images of cells treated with DMSO or DHPCC-9. Scale bars represent 20 μm. c MTT assays were used to determine the relative viability of PC-3 cells after 24 h treatments with PIM inhibitors. Shown are averages from three independent experiments with three parallel samples. d PC-3 cells were plated on coverslips and treated with DMSO or DHPCC-9 for 24 h, after which actin filaments were stained by phalloidin, and the number of actin protrusions and their length were measured. Shown are averages of indicated numbers (n) of cells along with e representative images for actin protrusions. Scale bars represent 10 μm. The increase in actin protrusion numbers in DHPCC-9-treated samples is pointed out by arrows