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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: RelB sustains endocrine resistant malignancy: an insight of noncanonical NF-κB pathway into breast Cancer progression

Fig. 6

RelB-mediated transcriptional regulation. mRNA expression profiles in the RelB-knocked out MDA-MB-231 cell vs. the parent cell were examined using a RNA-Seq platform. a The distribution of mRNA profiles in the RelB-deprived cell was illustrated. b Cluster analysis of RNA-Seq data indicates up/down-regulated mRNA profiles (heatmap). c KEGG pathway enrichment analysis was performed to assess EMT/metastasis relating mRNA profiles. d The protein level of MMP1 was quantified in the RelB-manipulated BCa cells. e A 5′-flanking region of the human MMP1 gene (5’MMP1) containing the NF-κB element (underline) and the core promoter region was cloned in pGL4 vector to drive the luciferase reporter gene expression as illustrated. The enhancer activity modulated by RelB in BCa cells was estimated by normalized reporter responses. f A small DNA fragment containing the NF-κB element was pulled down from chromatins using a RelB antibody and then quantified by PCR. IgG serves as a negative antibody control. Statistical significance between two groups as described in Fig. 3

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Cell Communication and Signaling

ISSN: 1478-811X