Fig. 6
From: The deubiquitinase USP22 regulates PD-L1 degradation in human cancer cells
USP22 targets CSN5 via deubiquitination. a HEK293FT cell lysates subjected to IP under non-denaturing conditions using either anti-HA or anti-His antibody. b IP assays of H1299 cells using anti-USP22 or anti-CSN5 antibody. c HEK293FT cells were transfected with indicated constructs. The impact of USP22 overexpression on CSN5 ubiquitination were tested. d The impact of CSN5 overexpression on USP22 ubiquitination was tested in HEK293FT cells. e In HEK293FT cells, wild type or loss of function mutation (C185A) of USP22 were transfected to measure CSN5 ubiquitination