Fig. 4
Fstl1-neutralizing antibody attenuates CCl4-induced liver fibrosis. a-c Babl/c mice were subjected to CCl4 along with Fstl1-neutralizing antibody 22B6 or isotype control IgG1 (25 μg/mouse) twice a week, and livers were harvested 4 weeks later for the following analyses (n = 6 mice per group). qRT-PCR analysis of α-SMA, Col1 and Fstl1 mRNA expressions. d Hydroxyproline contents in liver tissues were measured. e Sirius red staining of liver sections. Scale bars, 100 μm. f Protein expression levels of α-SMA, p-Smad2, Smad2, p-JNK, JNK in liver tissues were assessed by Western blot. Band intensity was quantified using Image J software and expressed as relative intensity compared with control. The ratio of α-SMA were subjected to GAPDH. The ratio of p-Smad2 was subjected to Smad2. The ratio of p-JNK was subjected to JNK. (G,H) Serum levels of ALT and AST were measured. Throughout, data represent means ± SEM. *P < 0.05, **P < 0.01