Fig. 5

RAPA promotes nuclear location of FOXO1 and PDGFB expression. a, b Western blot analysis of AKT, p-AKT, FOXO1, p-FOXO1, mTOR and p-mTOR on different groups of ECs treated with DMSO or rapamycin. c, d Expression of FOXO1 in the nucleus was detected by western blot. e NC-ECs, TIE2-WT-ECs and TIE2-L914F-ECs were subjected to IF staining with anti-FOXO1 antibody after the RAPA (15 nM) treatments for 48 h. f Average fluorescence intensity of each field was statistically analyzed by ImageJ software. g Expression levels of PDGFB were measured using real-time quantitative PCR. h Production of PDGFB released from the ECs was determined using ELISA. Scale bar, 50 μm. The data are means ± SEM (n = 3). ns: no significant, *P < 0.05, **P < 0.01