Fig. 2

BP ameliorates lung injury in CLP-induced polymicrobial sepsis. Mice were pretreated with BP for 2 h and lung tissues were collected 24 h after cecal ligation and puncture procedures. a to c The lung tissue hematoxylin and eosin staining (a, scale bar: 100 μm), lung injury score (b), and wet/dry weight ratio (c) were analyzed (n ≥ 5 per group). d Evans blue dye extravasation of the lungs was analyzed spectrophotometrically (n = 4 per group). e Immunoblot analysis of STAT3 and ERK1/2 expression in lung tissues (n = 3 per group). f The mRNA levels of the indicated genes were assayed by RT-PCR (n ≥ 5 per group; results are the mean ± SEM; one-way ANOVA test). (g to i) TF (G, green), PAR1 (h, green), MMP9 (i, green) and nuclei (blue) immunofluorescent staining of lung sections imaged by laser confocal microscope (n ≥ 3 per group; scale bar: 200 μm). j Laser confocal microscope images of Ki67 (green), F4/80 (red) and nuclei (blue) staining in lung sections (n ≥ 3 per group; scale bar: 200 μm). **P < 0.01 and *P < 0.05 (CLP versus Sham); ##P < 0.01 and #P < 0.05 (CLP versus CLP + BP); ns indicates not significant