Fig. 1

Expression of HOTAIR and c-Met in HCC cell lines and patient tissues through HCC progression. RT-qPCR analysis of (a) MET and (b) HOTAIR expressions in HCC cell lines FOCUS, SNU-449, SK-HEP-1, SNU-475, SNU-387, SNU-423, MAHLAVU, Hep-3B, HuH-7 and SNU-398. (c) RT-qPCR analysis of HOTAIR expression and immunoblotting of c-Met protein in HuH-7, Hep-3B, SNU-449 and SK-HEP-1 cells. (d) Normalized expression levels of MET and HOTAIR in normal, dysplasia, early and late HCC tissues in microarray dataset GSE89377. (e) Confocal microscopy image of fluorescent-in-situ hybridized HOTAIR mRNA and immunofluorescent labeled c-Met protein expression in control (MOCK) and HOTAIR over-expressing (HOTAIR OE) SNU-449 cells. RT-qPCR analysis of (f) MET and HOTAIR mRNA expression in MOCK and HOTAIR OE clones. RT-qPCR analysis of (g) HOTAIR and (h) c-Met mRNA expression in control si-RNA (NC) and si-HOTAIR transfected HuH-7 cells. Western blot analysis of (i) c-Met protein expression in control (NC) and si-HOTAIR transfected HuH-7 cells. Densitometric analysis of band intensities in immunoblots were analyzed with ImageJ and normalized with internal control protein band intensities. All graphs of experiments are presented as the mean ± SEM of at least 3 independent experiments. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001