Fig. 5

NEAT1 is upregulated and essential for ADV-induced GSCs formation by primary glioma cells. a Primary glioma cells were infected with ADV and cultured for 3 days. The expression of NEAT1 was determined by RT-qPCR, with β-actin as a reference control. b Primary glioma cells were infected with ADV and transfected with siRNAs to NEAT1, and then cultured under the neurosphere condition. The formation of tumorsphere was determined. c-e Primary glioma cells were infected with ADV and transfected with NEAT1 siRNAs, and then cultured for 3 days (NC, negative control. 1, siRNA-1. 2, siRNA-2). The expression of stemness-related transcription factors including c-MYC, SOX2, OCT4 and NANOG, was determined by RT-qPCR c and western blotting d, e, with β-actin as a reference control. Data are represented as mean ± SEM, n = 6. *, P < 0.05. **, P < 0.01. ***, P < 0.001