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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Identification of a novel IL-5 signaling pathway in chronic pancreatitis and crosstalk with pancreatic tumor cells

Fig. 4

IL-5Rα signaling activates Stat5 and promotes migration in PDAC cells. a Immunohistochemistry for IL-5Rα in increasing stages of pancreatic cancer in genetically modified mouse model (40x images). b Immunohistochemistry for IL-5Rα on tumors developed from orthotopically transplanted murine Panc02 (top) and human L3.6pl PDAC cells (bottom). c Immunofluorescent staining and confocal imaging for IL-5Rα (green), phalloiden (red), and nucleus (DAPI, blue). c Transwell assay analysis of total percent migration of 533 and L3.6pl cells in the presence of soluble IL-5 (0 or 200 ng/mL) (n = 3). d Confocal microscopy and MFI quantification for phospho-Stat5 (green) in L3.6pl cells treated with IL-5 for 5 or 15 min. Red phalloidin and blue DAPI shown in merged images (63x)

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Cell Communication and Signaling

ISSN: 1478-811X