Fig. 3

HA mediated the proliferation of MDA-MB-231BO-GFP cells in the matrix microenvironment. a. The HA content in the culture supernatant was assayed by CLIA. (****p < 0.0001). b. The growth of HS5 cells was detected by CCK-8 assays after adding 100 μmol/L, 300 μmol/L, 500 μmol/L, 700 μmol/L, and 1000 μmol/L 4-MU for 3 days. (**p < 0.01 and ***p < 0.005 compared with the control group as measured by unpaired Student’s t test). c. The growth of MDA-MB-231BO cells was detected by CCK-8 assays after adding 300 μmol/L 4-MU for 3 days. d. HS5 cells were pretreated with 300 μmol/L 4-MU. Twenty-four hours later, MDA-MB-231BO-GFP cells were added. Furthermore, treatment with 300 μmol/L 4-MU was continued in the coculturing system for 3 days. The HA content in the supernatants was determined by CLIA. (****p < 0.0001 compared with the control group as measured by unpaired Student’s t test). e. The fluorescence intensity of GFP was used to measure the growth of MDA-MB-231BO-GFP cells, which were separately treated with DMSO alone or cocultured with stromal HS5 cells before and after treatment with 300 μmol/L 4-MU. (*p < 0.05 and ****p < 0.0001 compared with the control group as measured by unpaired Student’s t test). Bars represent the mean ± SEM