Table 1 Primers used for real-time PCR
From: Gastrin-releasing peptide induces fibrotic response in MRC5s and proliferation in A549s
Gene | Primer sequences (5′ → 3′) | |
|---|---|---|
ACTA2 | Forward | CGAGATCTCACTGACTACCTCATGA |
Reverse | AGAGCTACATAACACAGTTTCTCCTTGA | |
VIM | Forward | GGACCAGCTAACCAACGACA |
Reverse | AAGGTCAAGACGTGCCAGAG | |
COL1A1 | Forward | CAAGAGGAAGGCCAAGTCGAG |
Reverse | TTGTCGCAGACGCAGATCC | |
TGFB1 | Forward | CGTGCTAATGGTGGAAACCC |
Reverse | GGTAGTGAACCCGTTGATGT | |
SMAD2 | Forward | CCAGGTCTCTTGATGGTCGT |
Reverse | TGGAGGCAAAACTGGTGTCT | |
SMAD3 | Forward | GCATGGACGCAGGTTCTCC |
Reverse | GGCTCGCAGTAGGTAACTGG | |
SMAD4 | Forward | GGAGGTGGCCTGATCTTCAC |
Reverse | CTTGGTGGATGCTGGATGGT | |
SMAD7 | Forward | CGGAAGTCAAGAGGCTGTGT |
Reverse | TGGACAGTCTGCAGTTGGTT | |
CTNNB1 | Forward | AAGTGGGTGGTATAGAGGCTCTTG |
Reverse | GATGGCAGGCTCAGTGATGTC | |
DKK1 | Forward | GCACCCAGGCTCTGCAGTCA |
Reverse | GCACGGGTACGGCTGGTAGT | |
GSK3B | Forward | CTCATGCTCGGATTCAAGCA |
Reverse | GGTCTGTCCACGGTCTCCAGTA | |
LEF1 | Forward | CATCAGGTACAGGTCCAAGAATGA |
Reverse | GTCGCTGCCTTGGCTTTG | |
AXIN | Forward | GAAGCGCGTGCGCATGGAGGA |
Reverse | GGCGGGAGGCAGCTTGTGAC | |
GRP | Forward | AGGTTCAAAAGGCAAAGGTTCT |
Reverse | GCAGAACGCAGTCTCTTAGG | |
GRPR | Forward | TGGCTAGACAGGAACCCTTG |
Reverse | CCTACACCACTCAGGAGCAT | |
HPRT | Forward | AAGGACCCCACGAAGTGTTG |
Reverse | GGCTTTGTATTTTGCTTTTCCA | |