Fig. 2
From: Gastrin-releasing peptide induces fibrotic response in MRC5s and proliferation in A549s
GRP does not induce epithelial-mesenchymal transition in A549 cells. Figure shows protein bands of α-SMA and E-cadherin (a), and the alterations at the protein levels of α-SMA (b) and E-cadherin (c). GRP induces the differentiation into myofibroblasts and the production of ECM components in MRC5. Figure shows protein bands of α-SMA and Fibronectin (d), the alterations at protein levels of α-SMA (e) and fibronectin (FN) (f), the immunoreactivity at 0-72 h periods of α-SMA (green) and Collagen 1α1 (red) (g), and the changes at gene expression of ACTA2, VIM, COL1A1(h). P values are *p < 0.05 and **p < 0.01 compared to Time (0 h); +p < 0.05 and ++p < 0.01 compared to Time (24 h); #p < 0.05 and ###p < 0.001 compared to Time (48 h). Original magnification ×400; scale bar, 50 μm