Table 1 Primer sequences used in this study
Assay | Gene | Sequence | ||
|---|---|---|---|---|
shRNA | p53 (position 775 to 794) | Forward | 5′-GATCCGGACTCCAGTGGTAATCTACTTTCAAGAGAAGTAGATTACCACTGGAGTCTTTTTTG-3’ | |
Reverse | 5′-AATTCAAAAAAGACTCCAGTGGTAATCTACTTCTCTTGAAAGTAGATTACCACTGGAGTCCG-3’ | |||
Mutagenesis | p53 mutant type (R248Q) | Forward | 5′-ATGAACCaGAGGCCCATCCTCACCATCATCA-3’ | |
Reverse | 5′-GGGCCTCtGGTTCATGCCGCCCATGCA-3’ | |||
mRNA | Fibronectin 1 | Forward | 5′- CCATCGCAAACCGCTGCCAT-3’ | |
Reverse | 5′-AACACTTCTCAGCTATGGGCTT-3’ | |||
Promoter | Fibronectin 1 (−2028 to − 23) | Forward | 5′-CGGCTAGCTTCAGTGCAGTAAATATATC-3’ | |
Reverse | 5′- ATCTCGAGTTATATGGGACGGTCCCCTCCCGCC-3’ | |||