Fig. 4
Relationship between p53-KD and cancer stem cell features or cell migration. a Aldefluor analysis in OV-shp53 and control cells. Note the R1 populations including the ALDHhigh population with cancer stem cell-like features. b Left: wound-healing assay with OV-shp53 and control cells. A scratch ‘wound’ was introduced to the middle of wells containing cell growth to confluency, and phase contrast images were taken after 6, 9, and 12 h. Right: the values of wound areas in 0 h were set as 1. The fold wound areas are presented as means±SDs. C, control. c Migration rate measured using transwell assay. Left: the OV-shp53 and control cells were seeded in a 24-well transwell plates and incubated for 24 h in medium without serum. Cells were stained with HE and counted using a light microscope. Right: the numbers of migrated cells are presented as means±SDs