Fig. 3

Relationship between p53-KD and cell proliferation or apoptosis. a Left: two independent OV-shp53 and control cell lines were seeded at low density. The cell numbers are presented as means±SDs. P0, P3, P6, and P8 are 0, 3, 6, and 8 days after seeding, respectively. Right: FACS analysis of OV-shp53 and control cells at 3 days after seeding (P3). b Western blot analysis for the indicated proteins in total lysates from OV-shp53 and the mock cells. c Left: after treatment with 10 μM CDDP, OV-shp53 and control cells undergoing apoptosis are indicated by arrows. Original magnification, × 400. Right: the numbers of apoptotic cells are demonstrated as means±SDs. Con, control. d Upper: treatment of OV-shp53 and control cells with 10 μM CDDP for the times shown. Cell viability was measured using the CCK-8 kit. The viability in the absence of CDDP treatment (0 h) is set as 100%. Lower: treatment of OV-shp53 and control cells with 10 μM CDDP for the times shown. The numbers of trypan blue-positive cells (non-viable cells) are presented as mean ± SD. This experiment was performed in triplicate using independent samples. e Western blot analysis for the indicated proteins in total lysates from OV-shp53 and control cells treated with 10 μM CDDP