Fig. 1

Expression of HNF-1β and ARID1A in p53-KD cells. a Western blot analysis for the indicated proteins in total lysates from OV-shp53 and control cells (Con). b Analysis of endogenous HNF-1β mRNA expression by conventional (upper) and real time RT-PCR assay (lower) for OV-shp53 and control cells (Con). The fold changes in mRNA expression detected by real time RT-PCR are presented as means±SDs. The experiment was performed in triplicate. c OVISE cells were transfected with two HNF-1β reporter constructs, respectively, together with either p53wt or p53mt. Relative activity was determined based on arbitrary luciferase light units normalized to pRL-TK activity. The activities of the reporter plus the effector relative to that of the reporter plus empty vector are shown as means±SDs. The experiment was performed in duplicate