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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Autophagy suppression of trophoblast cells induces pregnancy loss by activating decidual NK cytotoxicity and inhibiting trophoblast invasion

Fig. 2

Autophagy in trophoblasts restricts the cytotoxicity of dNK cells. a The levels of autophagy-related proteins LC3B and P62 were detected by western blot. b, c HTR-8/SVneo cells were pretreated with rapamycin, then co-cultured with dNK (n = 9) cells for 48 h. The expression of CD16, NKG2D, NKP30 and NKP46 in dNK cells were detected by FCM. d The inhibitory effect of 3-MA on autophagy was verified by western blot. e, f HTR-8/SVneo cells were pretreated with 3-MA and then co-cultured with dNK (n = 9) cells for 48 h. The expression of CD16, NKG2D, NKP30, NKP46, CD107, IFN-γ and Granzyme B in dNK cells were detected by FCM. Data are expressed as the mean ± SEM; paired t-test or Kruskal-Wallis test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, NS: no significance

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Cell Communication and Signaling

ISSN: 1478-811X