Table 1 Reagents and antibodies
From: CAMKK2-CAMK4 signaling regulates transferrin trafficking, turnover, and iron homeostasis
Name | Description | Source | Catalogue number |
|---|---|---|---|
Total Iron assay kit | Spectrometric measurement of total iron content | Sekure Chemistry | 102–25 |
CAMKK2-CRISPR/Cas9 plasmid | gRNA and tracrRNA constructs | SCBT | SC-400928 |
CAMKK2-HDR plasmid | 5′ and 3′ HDR sequence flanking a floxed puromycin/RFP reporter cassettes | SCBT | SC-400928-HDR |
CAMK4-CRISPR/Cas9 plasmid | gRNA and tracrRNA constructs | SCBT | SC-400806 |
CAMK4-HDR plasmid | 5′ and 3′ HDR sequence flanking a floxed puromycin/RFP reporter cassettes | SCBT | SC-400806-HDR |
Anti-TF (for human) | Mouse monoclonal (Clone-E8) | SCBT | SC-393595 |
Anti-TF (for mouse) | Mouse monoclonal (Clone-F8) | SCBT | SC-373785 |
Anti-TFRC (CD71) | Mouse monoclonal (Clone-2B6) | SCBT | SC-51829 |
Anti-CAMKK2 | Mouse monoclonal (Clone-ZZ9) | SCBT | SC-100364 |
Anti-CAMK4 | Mouse monoclonal (Clone—A3) | SCBT | SC-166256 |
Anti-GAPDH | Mouse monoclonal (Clone-0411) | SCBT | SC-25778 |
Anti ERK1/2 | Rabbit polyclonal | CST | 9102 |
DNA sequencing primers | |||
Primer name | Sequence (5′-3′) | ||
Camkk2 Forward | TAA AGA CCA TGA TTC GAA AG | ||
Camkk2 Reverse | CTT TCA CAA GAG CAC TTC | ||