Fig. 6
From: CAMKK2-CAMK4 signaling regulates transferrin trafficking, turnover, and iron homeostasis
Effect of CAMKK2 and CAMK4 loss on the charged fractions (PTMs) of TFRC during TF trafficking. a-h: Immunoblots showing IEF/SDS-PAGE-based charge-separated TFRC in serum-starved wild-type (AE), CAMKK2−/− (BF), CAMK4−/−(CG) and DKO (DH) HEK293 cells. Cells were transiently transfected with RFP-TFRC for 6 h and then treated with or without 25 μg/ml TF for 30 mins. I-L: The immunoblots in A-D and E-H were false-colored and overlaid to show the relative shift of TFRC charged fractions following TF treatment. The red rectangle indicates a major fraction of the TFRC at pH 6–7 (TFRC pI: 6.18). The green rectangle indicates acidic fractions (pH ~ 3–4). HMW: High molecular weight fraction.