Fig. 3
CLPTM1L expression is positively associated with the ERβ-induced genes in NSCLC. a Venn diagram showing the overlapping target genes of CLPTM1L, ERβ, and IR. b Real-time PCR analysis of the expression of 16 candidate target genes in A549 cells treated with CLPTM1L siRNAs/control siRNA and IR. c CLPTM1L binding to the promoters of CDC25A, c-Jun, and BCL2 was examined by ChIP-qPCR assay in A549 cells exposed to IR. d The expression levels of CLPTM1L, CDC25A, c-Jun, and BCL2 were detected by IHC staining in 110 cases of NSCLC tissue samples using tissue microarray. Representative images were taken from the same sample in 110 cases of the tissue microarray (see the No.55 case in Additional file 1: Table S11). e–g Real-time PCR analysis of the correlation between CLPTM1L and CDC25A (or c-Jun and BCL2) mRNA levels in seven NSCLC cell lines exposed to IR (CDC25A, P < 0.01, R2 = 0.8876; c-Jun, P < 0.01, R2 = 0.8302; BCL2, P < 0.01, R2 = 0.7850; Pearson’s correlation). All experiments were repeated at least three times. Statistically significant differences are indicated. Student’s t test; ** P < 0.01