Fig. 5

Inhibition of β-catenin downregulates C/EBP and NF-κB activity in NHAs. a NHAs were transfected with C/EBP-β, C/EBP-δ, RelA, or scrm siRNA and at 48 h, their respective mRNA expression was measured by real-time PCR. b NHAs were transfected with C/EBP-β, C/EBP-δ, Rel A, or scrm siRNA and at 24 h co-transfected with C/EBP or NF-κB reporter plasmids and 24 h later luciferase reporter activity was measured. c NHAs were transfected with C/EBP-β, C/EBP-δ, RelA, or scrm siRNA and at 48 h IL-6 mRNA expression was measured by real-time PCR. d NHAs were transfected with β-catenin, TCF1, TCF3, TCF4, LEF1, and scrm siRNA for 24 h then transfected with C/EBP or NF-κB reporter plasmid for another 24 h followed by luciferase reporter activity measurement at 48 h post transfection. * indicates p ≤ 0.05 in comparison to respective control. The data is presented as mean ± SEM (n = ≥3, one-way ANOVA, post hoc Dunnett’s)