Fig. 4
From: β-Catenin and TCFs/LEF signaling discordantly regulate IL-6 expression in astrocytes
Role of TCF/LEF alternative transcriptional partners (ATF2 and Smads) in IL-6 gene regulation. a NHAs were transfected with siRNA for ATF2, Zeb1, or scrambled (scrm) siRNA (a, b, respectively) or treated with Smads 2 and 3 inhibitor (SB525334) or Smads 1, 5, and 8/9 inhibitor (c and d respectively). Target gene as indicated was measured at 48 h by real-time PCR in the siRNA KD experiments (a, b) and at 24 h post small molecule inhibitor experiments (c, d). * indicates p ≤ 0.05 in comparison to control. The data is presented as mean ± SEM (n = 3, one-way ANOVA, post hoc Dunnett’s performed in (a), (b) and one sample T-test performed in (c) and (d))