Fig. 1
Effects of estrogen on PIWIL1 expression in endometrial cancer cells. a,b,c Ishikawa, RL95–2 and HEC-1B cells were treated for treated for different times(24 h, 48 h, 72 h) with different concentrations of E2 (10− 10 ~ 10− 8 mol/L). PIWIL1 mRNA and protein levels were measured by RT-qPCR and western blot. Data were represented as means ± SD for three independent experiments (RT-qPCR). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 and ns, not significant versus control group. GAPDH was used as an internal control (western blot)