Fig. 7

Blockade of Cx43-mediated hemichannel ATP release, negates the TGF-β1-induced disassembly of the tight and adherens junction complex. hPTECs were cultured in low glucose (5mM) ± TGF-β1 (10 ng/mL) ± Peptide 5 (25 μM) for 48 h. Expression of E-cadherin (a), N-cadherin (b), Claudin-2 (c) and ZO-1 (d) were assessed via western blotting. Representative blots for each protein are shown, with expression normalized by re-probing for ɑ-tubulin as a loading control. Bars correspond to their associated lanes in the respective blot. Results were from three or more separate experiments; with significance shown: *P < 0.05; **P < 0.01