Fig. 5

Blockade of purinoreceptors attenuates TGF-β1 induced changes in expression of adherens and tight junction protein. HK2 cells were cultured in low glucose (5mM) ± TGF-β1 (10 ng/mL) ± either: Suramin (100 μM), A438079 (50 μM) or A804598 (50 nM) for 48 h. Whole-cell expression of E-cadherin (a), N-cadherin (b), β-catenin (c), Claudin-2 (d) and ZO-1 (e) were assessed via immunoblotting. Representative blots for each protein are shown, with expression normalized by re-probing for ɑ-tubulin as a loading control. Bars correspond to their associated lanes in the respective blot. Results were from three or more separate experiments; with key significance shown: *P < 0.05; **P < 0.01; ***P < 0.001